Cobalt Chelating Superflow [EQUL-BSM19] Equl offers Enzyme Assay Kits, Reagent Mixtures, Enzymes, Glycobiology, Amylase Test, Carbohydrase Tablet Tests, Protease Tablet Tests, Cofactors and Stains, Soluble Chromogenic Substrates, Insoluble Chromogenic Substrates, etc 

Cobalt Chelating Superflow

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EQUL-BSM19 Cobalt Chelating Superflow EA call for price none
Product Code37409
Bead Size60-160µm
Agarose concentration6%
Flow rate1400cm/h*
Binding Capacity5-10mg/ml of 6X His-tagged protein
Cleaning0.5M NaOH for 30min
Storage Conditions20% ethanol, 2-8C
Reactive group concentration26 µmole Co/ml gel
Temperature stability range0-60 C
Functional ligandProprietary
pH stability range2-13(<2hr) or 3-12 (>2hr)
Chemical stabilityHigh salt, 0.5M NaOH (<2hr), uea, guanidine, organic solvents: ethanol (70%), methanol (50%)
Drug master fileU.S. Food & Drug Administration

*Flow rate determined in a 1.5x5cm column

- For binding of His-tagged proteins
- High binding capacity with low non-specific binding
- Performance is on par with leading cobalt-chelating resins
- Immobilized on Sterogene’s own patented Superflow matrix (Most industry leading chelators use Sterogene’s Superflow matrix.)
- Cleanable, rechargeable and industrial process-ready
- No detectable leaching
- cGMP manufacturing; Regulatory Support File available
- DMF with USFDA

Cobalt Chelating Superflow is an immobilized metal affinity chromatography (IMAC) resin that separates proteins via their differences in affinity to cobalt. This product is recommended for clarified feed streams.

A number of commercially available metal-chelating resins differ by how strongly they bind a given metal. For instance, Sterogene’s IDA Chelating Ultraflow and IDA Cellthru BigBead are tri-dentate and typically have very high binding capacities in comparison to other chelators. However, relative to tetra-dentate chemistries, tri-dentate chelators tend to have higher metal leakage properties

NTA is a tetra-dentate chelator, leaving two coordination sites available on a transition metal to bind chelating moieties on proteins (such as histidine repeats). Cobalt Chelating Superflow features a proprietary tetra-dentate chelating chemistry (not NTA) optimized for binding His-tagged proteins with cobalt. Sterogene’s proprietary IMAC chemistry maintains excellent binding capacities while mitigating leaching and nonspecific binding properties.

The product is shipped pre-charged and ready to use. While optimizing IMAC separations usually requires some method development, Cobalt Chelating Superflow is designed to be a drop-in replacement for leading cobalt resins