PureCol® collagen is known as the standard of all collagens for purity (>99.9% collagen content), functionality, and the most native-like collagen available. PureCol® is isolated from bovine hides sourced from the only controlled, closed herd in the United States. Advanced BioMatrix’s manufacturing processes comply with stringent quality standards that have proven to yield unsurpassed lot-to-lot consistency.

PureCol® collagen is approximately 97% Type I atelocollagen with the remainder being comprised of Type III collagen. PureCol® collagen is supplied at approximately a 3 mg/ml concentration. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product. PureCol® is soluble atelocollagen in 0.01 N HCI, therefore, the pH is 2. PureCol® collagen is ideal for coating of surfaces, providing preparation of thin layers for culturing cells, or use as a solid gel.

PureCol® collagen is provided in a user-friendly packaging for use and storage. PureCol® is sterile filtered and is supplied as a ready to use solution.

Parameter, Testing, and Method	PureCol® Type I Collagen #5005
Sterilization Method	Filtration
Extraction Method	Enzyme - atelocollagen
Form	Solution
Package Size	100 mL, 1000 mL
Storage Temperature	2-10°C
Shelf Life	Minimum of 6 months from date of receipt
Collagen Concentration - Biuret	2.9-3.2 mg/mL
Collagen Purity - Silver Staining	>99.9%
pH	1.9-2.1
Kinetic Gel Test (Minutes)	<40
Gel Formation Tube Test (Minutes)	< 40
Fibrillogenesis (Absorbance Units)	>0.5
Electrophoretic Pattern - Coomassie Blue	Characteristic
Sterility - USP modified	No growth
Endotoxin - LAL	<1.0 EU/mL
Osmolality (mOsmo H2O/kg)	<35
Cell Attachment Assay	Pass
Source	Bovine Hide
Hydrogel Young's Modulus E (Pa)	Characteristic