Actigel ALD

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EQUL-BSM04 Actigel ALD EA call for price none
Product Code2701
Bead Size60-160um
Agarose concentration4%
Spacer arm5 atoms, hydrophilic
Flow rate300cm/hr*
Binding capacityUp to 60mg/ml
Cleaning0.15 IM NaOH**
Storage Conditions20% ethanol, 2-8C
Functional to attachPrimary amines
Reactive group concentration>50umoles/ml
Coupling effeciencies>90%
Temperature stability range2-100 C (wet)
Functional ligandMonoaldehyde
Blocking agentsEthanolamine***
Coupling in organic solventsYes
Coupling in a packed columnYes
pH stability range2-13
Coupling conditionspH 3-10, 0-40C, 2-12 hours
Chemical stability1M NaOh, 8M urea, 6M guanidine, ethanol, and common buffers..
Drug master fileU.S. Food & Drug Administration

*Flow rate determined in a 1.5x5cm column
**For uncoupled resin; once active groups are used, the resin can withstand up to 1M NaOH. Please note, though, that your immobilized ligand may not withstand this concentration
***Blocking is not usually necessary.

-For stable immobilization of proteins through the primary amine group
-Monoaldehyde activation provides superior performance compared with other aldehyde activations such as epichlorohydrine/sodium periodate and glutaraldhyde methods
-High subsitution levels: immobilizations reach up to 60mg immobilized pretein per ml resin
-Cleanable and industrial process-ready
-Utitlizes Sterogene's proprietary Uniflow 4 matrix (standard)
-Other available supports for Actigel include Superflow, Ultraflow 4 and Ultraflow 6
-Cellthru 300 and Cellthru BigBead are also inventoried with Actigel ALD chemistry
-cGMP manufacturing; Regulatory Support File available
-Actigel ALD is an activated resin used to immobilize primary amines from small ligands to large

During coupling, an instantaneous, reversible Schiff base reaction takes place between the monoaldehyde groups and the primary amines of the ligand. The Schiff base is then reduced to a stable secondary amine by the addition of a reducing agent so mild that it does not reduce the intramolecular disulfide bridges within the immobilized protein. ALD couples both acidic and basic proteins with the same high efficiency, typically >90% due to the high density of stable aldehyde groups.

Coupling is highly reproducible. Using ALD chemistry, coupling efficiency is simply a function of loading. No hydrolysis or inactivation of active groups takes palce during the coupling reaction. This reproducibility is critical for both research and manufactoring. (Typically, hydrolysis or inactivatoin of the activated groups is the main cause of variable coupling efficiency encountered with traditional coupling chemistries.)

The monoaldehyde functional gorup of Actigel also avoids one of the major drawbacks of glutaraldhyde adn periodate activations: cross-polymerizatoin due to polymeric aldehyde groups that can affect the reproducibility of a coupled matrix.

Actigel ALD is available on a variety of agarose supports, each with its own applications and adavantages. For instance, higher flow rates can be achieved with Actigel Superflow adn Actigel Ultraflows (4% and 6%). Alternatively, Activated ALD Cellthru 300 and Activated ALD Cellthru BigBead can be used with vicous and crude feedstreams taht are otherwise unsuitable for standardiezed beads (60-160um).

Also Consider
Sterogene's customization service, immobilization services, and Do-It-Youself kits of resin optimization programs.