Wheat Germ Agglutinin [ML-WGA20] - $183.04 Equl offers Enzyme Assay Kits, Reagent Mixtures, Enzymes, Glycobiology, Amylase Test, Carbohydrase Tablet Tests, Protease Tablet Tests, Cofactors and Stains, Soluble Chromogenic Substrates, Insoluble Chromogenic Substrates, etc 

Wheat Germ Agglutinin

Price(USD):$190.71  $183.04

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Equl SKU Product Name UOM Specification Price(USD) Add to Cart:
ML-WGA20 Wheat Germ Agglutinin EA 20 mg $190.71  $183.04  
Introduction:

PROPERTIES

(1)FORM
Off-white lyophilised powder (affinity purified).

(2)SPECIFICITY
WGA is a non-blood group specific lectin.It has an affinity for oligomers of N-acetyl glucosamine with activities being reported as GlcNac < (GlcNAc)2< (GlcNAc)3 = (GlcNAc)4 < (GlcNAc)5a.

(3)ACTIVITY
WGA at a concentration of 20 µg/mL gives a 50 % agglutination of a 2 % suspension of human red blood cells [type O(H)].Incubations performed for 1 h at room temperature in phosphate saline buffer (PBS).*

(4)PROPERTIES
UV absorbance (in 10 mM phosphate buffer,pH 7.0) lmax= 275.8;lmin= 250.5 nm
Solubility:1 mg/mL in PBS (pH 7.2);~ 1 mg/mL in 1 mM Tris/HCl (pH 7.4);
> 10 mg/mL in 100 mM acetic acid.

(5)STORAGE / STABILITY / SAFETY
Storage temperature:2 - 8oC.(or -20oC)
Shelf life:> 3 years (at -20oC).
Safety statement:Please refer to the MSDS sheet for this product on the Megazyme website.

(6) ELECTROPHORETIC PURITY
- single major band band on SDS-gel electrophoresis (MW = 18,000);several very minor bands.
- reportedbpI of 8.7 +0.3 for isolectins I,IIa and III;and pI 7.7 +0.3 for isolectin IIb.

Figure 1.SDS-PAGE analysis of WGA.Electrophoresis wasperformed using a 14 % acrylamide gel.
lane A,low molecular weight markers (in-house standards);
lane B,Competitor WGA,10 µg;
lane C,Megazyme WGA,10 µg;
lane D,low molecular weight markers (in-house standards).