RANDOX Tramadol ELISA

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Introduction:

Tramadol is a synthetic opioid-receptor agonist that has been used clinically as a narcotic analgesic since 1977. Tramadol also inhibits the reuptake of monoamines such as norepinephrine and serotonin, which enhances its analgesic effect. O-desmethyltramadol has 2-4 times the analgesic efficacy of the parent drug. In all body tissues and blood the drug concentration is greater than the N-desmethyl and O-desmethyltramadol metabolites, and O-desmethyltramadol is always greater in concentration than the N-desmethyl metabolite. There are numerous reports of tramadol toxicity and abuse. Overdoses of ≥500 mg may cause agitation, hypertension, tachycardia and seizures, while doses >800 mg may cause coma and respiratory depression.

Cross Reactivity

Analyte Compound
Cross Reactivity %
Tramadol
LOD: 0.86 ng/mL
Tramadol 100
O-desmethyltramadol (Hydroxytramadol)
57
N-desmethyltramadol (Nortramadol)
4
 
rac N,O-didesmethyltramadol (Norhydroxytramadol)
1
 
Phencyclidine (PCP)
<0.1