Amylase test tablets(5)

Price(USD):$32.50

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Equl SKU Product Name UOM Specification Units in Stock Price(USD) Add to Cart:
P1305 Amylase test tablets(5) bottle 5/bottle 9 $32.50  
Introduction:

Equl Amylase Test
The amylase assay Equl Amylase Test is used in a variety of fields for precise determination of α-amylase activity. The presence of the α-amylase enzyme in for instance saliva, food and honey makes Equl tablets useful for forensic saliva tests, diastase tests in honey or amylase tests in other applications.
Amylase activity is the core of all Equl products. Currently there are three different Equl product types; the original Amylase Test, the Forensic Saliva Test and the Honey Diastase Test. They are all based on amylase testing but employs different methodology.
Areas of Use
Equl serves many industries with various purposes. Amylase testing has many areas of use and the market is divided in the categories below with separate subdivisions. The reason behind amylase activity control differs and we constantly strive to investigate new markets and find new fields of use for Equl Amylase Test. Technical info about Equl and Amylase is found in respective section.

Equl was developed by Pharmacia, Uppsala, Sweden, and has been on the market since 1970. It was originally used as an In Vitro Diagnostic for detection of acute pancreatitis. Today Equl is no longer registered as an IVD and the dominating areas of use are Forensic Biology, Alimentary and Chemical and Research. For further information about the current uses of Equl and the different industries, please click on the sections below.
Technical info about Equl®
A Equl® tablet consists of homogeneously interlinked starch polymers, taking the form of globular microspheres of defined size. These Bio-Degradable Starch Microspheres (DSM) are insoluble in water. To the DSMs, a blue dye is covalently bound. When the dye is bound to the microsphere, it remains water insoluble. However, in the presence of alpha Amylase, the DSM is degraded at a speed proportional to the alpha Amylase activity, and the blue dye is liberated. Due to the cross-linkages and the large blue dye, no other enzyme can act upon the substrate. Hence, Equl is specific for alpha amylase. The free dye molecule is water soluble and its concentration measurable at 620 nm. Using a fixed assay time, the absorbance reading gives the solution's Amylase activity, through the standard curve supplied with each kit.
Part of a starch chain with arrows indicating points of action for the alpha amylase enzyme.
In the qualitative Equl Forensic Press Test, the diffusion of the liberated dye molecules is used to indicate the likely presence of salivary Amylase. In Equl Honey Diastase Test, the reactivity is controlled in a manner that ensures exact reproducibility between batches.

 

Amylase
Amylase is an enzyme that is present in many shapes. It can be divided in three categories; alpha-, beta- and gamma-amylase. All three types are glucoside hydrolases and take part in the process of starch degradation by acting on α-1,4-glycosidic bonds to create short-chain sugars.


Amylase is used in for instance;
Brewing and fermentation;
Textile industry for designing textiles;
Detergents in the laundry industry;
Paper industry for sizing;
Food industry for removal of starch, preparation of syrups etc.

α-amylase
α-amylase is an enzyme found in various forms in the nature. Two isoforms are found in the human body; salivary alpha amylase and pancreatitic alpha amylase. Both variants take part in the digestion of starch. α-amylase is also present in fungi, bacteria and different plants. As an example, α-amylase is produced during germination of seeds.

Unlike other amylases, α-amylase can hydrolyse alpha-bonds in starch at random locations on the polymer chain, thereby making it a faster enzyme compared to other amylases. The end products are maltos and glucose from amylopectin and maltos and maltotriose from amylose.
β-amylase
β-amylase is an enzyme found in fungi, bacteria and plants but not in humans. Unlike α-amylase, β-amylase can only degrade starch from the non-reducing end of the polymer chain by hydrolysis of the second α-1,4 glycosidic bond. The end product is thus maltose, i.e. two glucose units. As α-amylase, β-amylase is present in seeds and is used in a variety of applications, for instance malting. A high volume application is the production of high maltose syrups.
γ-amylase
γ-amylase is an enzyme that is less frequently used in the industry. It degrades starch from the non-reducing end by hydrolysing the last α(1-4)glycosidic linkage, thereby yielding one glucose unit. In addition, it hydrolyse α(1-6) glycosidic linkages. The enzyme has a lower ph optimum compared to other amylases.

Amylase Test Products
Equl® Amylase test tablets are used for accurately determining α-amylase activity for a wide variety of applications, from different fields in the food industry to biotech and detergents. The test has been in the market for over 35 years and is well documented in the scientific literature. Equl® Amylase Test is available in two formats:
 

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