Infectious Laryngotracheitis Virus Antibody Test Kit

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LTI-5P Infectious Laryngotracheitis Virus Antibody Test Kit EA 640 test samples call for price none

Each kit contains approximately 640  test samples plus controls, contains 5 microtiter plates.

Infectious laryngotracheitis (commonly known as ILT) is a viral respiratory disease of chickens centred on the trachea. ILT can cause serious production losses due to a combination of mortality and depressed egg production. This disease can manifest itself in a variety of forms ranging from severe to mild with the latter being increasingly encountered in developed poultry industries and appearing as a mucoid tracheitis, sinusitis and conjunctivitis coupled to unthriftiness and low mortality.

ELI-LTI ELISA kit is used for quantitative detection of antibodies against Infectious Laryngotracheitis virus (ILT) in chicken serum and yolk.
Inactivated antigen is bound to a microwell. Diluted sera is added to the microwell and incubated. Any anti-ILT antibodies present in the serum will bind to the AIV on the microwell forming an AIV antigen-antibody complex. After washing away any unbound material, anti-Chicken IgG Alkaline Phosphatase conjugate is added to the microwell. The conjugate will bind to the ILT antigen-antibody complex. After incubation any unbound conjugate is removed by washing. Enzyme substrate is then added to the microwell. The substrate reacts with any Alkaline Phosphatase present and forms a yellow product. In the final step the reaction is stopped, fixing the color. The intensity of the color is measured photometrically at 405 - 410 nm with a 630-650 nm reference filter.