Foot & Mouth Disease Virus

Price(USD):$812.50

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EQUL-FMD-PCR Foot & Mouth Disease Virus EA $812.50  
Introduction:

    Foot-and-mouth disease, FMD or hoof-and-mouth disease (Aphtae epizooticae) is a highly

 

contagious and sometimes fatal viral disease of cloven-hoofed animals, including domestic

 

animals such as cattle, water buffalo, sheep, goats and pigs, as well as antelope, bison and

 

other wild bovids, and deer. It is caused by foot and Mouth disease virus.

 

Humans are very rarely affected by the virus. Foot and Mouth disease virus is the prototypic

 

member of the Aphthovirus genus in the Picornaviridae family. This picornavirus is the

 

etiological agent of the acute systemic vesicular disease that affects cattle and other animals

 

worldwide. It is a highly variable and transmissible virus.

 

There are seven FMD serotypes: O, A, C, SAT-1, SAT-2, SAT-3, and Asia-1. These serotypes

 

show some regionality, and the O serotype is most common.

 

 

 

Kit storage and stability

 

This kit is stable at room temperature but should be stored at -20ºC on arrival.

 

PrimerDesign does not recommend using the kit after the expiry date stated on the pack.

 

Once the lyophilized components have been re-suspended, unnecessary repeated

 

freeze/thawing should be avoided. The kit is stable for six months from the date of

 

resuspension under these circumstances.

 

Suitable sample material

 

All kinds of sample material suited for PCR amplification can be used. Please ensure the

 

samples are suitable in terms of purity, concentration, and RNA/DNA integrity (An internal

 

PCR control is supplied to test for non specific PCR inhibitors). Always run at least one

 

negative control with the samples. To prepare a negative-control, replace the template RNA

 

sample with RNAse/DNAse free water.

 

Dynamic range of test

 

Under optimal PCR conditions PrimerDesign genesig detection kits have very high priming

 

efficiencies of >95% and can detect less than 100 copies of target template.

   

 

    Kit Contents
• FMDV specific primer/probe mix (150 reactions BROWN)
FAM labeled, BHQ quenched
• FMDV positive control template (for Standard curve RED)
• FMDV RT primer mix (150 reactions GREEN)
Required for two step protocol only
• RNAse/DNAse free water
Reagents and equipment to be supplied by the user
Real-Time PCR Instrument
RNA extraction kit
This kit is designed to work well with all processes that yield high quality RNA with minimal
PCR inhibitors.
Precision OneStepTM qRT-PCR MasterMix kit
Contains complete one step qRT-PCR MasterMix plus Standard MasterMix for no template
control
Optional - Reverse Transcription kit and Mastermix
Although a one step RT-PCR protocol is recommended, this kit is designed to work well
with a two step protocol. We recommend the use of PrimerDesign PrecisionTM Reverse
Transcription kits and the use of PrimerDesign 2x PrecisionTM MasterMix for the two step
protocol.
Pipettors and Tips
Vortex and centrifuge
Thin walled 1.5 ml PCR reaction tubes