HyStem-HP®, Hydrogel Kit

Price(USD):$316.94

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BMGS315-1EA HyStem-HP®, Hydrogel Kit EA 1 Kit $316.94  
Introduction:

HyStem-HP™ is recommended for stem cell researchers whose applications require slow release of growth factors in a cell’s microenvironment. HyStem-HP contains small amounts of thiolated heparin which ionically binds a wide variety of growth factors and slowly releases them over time1,2,3.

HyStem-HP is ideal for stem cell applications where slowly released growth factors are crucial in recreating a stem cell niche.  HyStem-HP™ hydrogels contains thiol-modified heparin which allows the slow release of growth factors (GFs) within an easily customizable, environment. HyStem-HP™ is a synthetic extracellular matrix (ECM) that can be injected and crosslinked in situ. Unlike an animal-derived extracellular matrix (ECM), HyStem-HP™ is chemically defined and nonimmunogenic. The HyStem-HP™ Hydrogel Kit contains HyStem-HP™ (a combination of thiol-modified hyaluronan, HA, and thiol-modified heparin), Gelin-S™ (thiol-modified gelatin), and Extralink™ (a thiol-reactive crosslinker, polyethylene glycol diacrylate, PEGDA).

The immobilized heparin in the hydrogel mimics the heparan sulfate proteoglycans normally present in the extracellular matrix (ECM). It also helps protect GFs from proteolysis and slows their release to attached cells1. This reduces the amount of GF required to achieve stimulation of cell growth or differentiation when compared to the use of free GF in media. All GFs tested to date (bFGF, VEGF, Ang-1, PDGF, TGFβ1, KGF) are released at different rates, but over a period of several weeks1,2,3.

Gelation

Reconstituted HyStem-HP™ components remain liquid at 15 to 37°C. The hydrogel is formed when Extralink is mixed with HyStem-HP™ and Gelin-S™. Gelation occurs about twenty minutes after all three components are mixed. No steps depend on low temperature or low pH. Diluting the components with phosphate-buffered saline (PBS) or cell-culture medium can increase gelation time.

Volume and Composition

HyStem-HP™ Hydrogel Kits comes in one size:

  • 7.5 ml with three sets of vials that make 2.5 ml each (for small-volume applications)
  • 3x 1.0 ml of HyStem-HP, 3x 1.0 ml of Gelin-S™, 3x 0.5 ml of Extralink

Flexibility

  • HyStem-HP™ ensures complete control over:
  • amount and type of GF incorporated
  • cell encapsulation or plating on top of hydrogel
  • cell-growth format (96- to 6-well plates and/or tissue-culture inserts)
  • amount and type of ECM proteins incorporated
  • hydrogel stiffness

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