VitroCol®, Human Collagen, Lyophilized, 15 mg

Price(USD):$338.00

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Equl SKU Product Name UOM Specification Price(USD) Add to Cart:
BM-5008-15ML VitroCol®, Human Collagen, Lyophilized, 15 mg EA 15 mg $338.00  
Introduction:

VitroCol®collagen is the first widely available, naturally produced purified human collagen for research purposes. VitroCol® sets the standard for purity (>99.9% collagen content), functionality and represents the only native-like human collagen offered.

VitroCol® collagen is naturally secreted from human neo-natal fibroblast cells. The human fibroblasts are cultured in optimal conditions allowing the fibroblasts to naturally and efficiently secret extracellular matrix. The extracellular matrix is then processed and purified to yield the naturally produced human collagen.

VitroCol® is approximately 97% Type I human collagen with the remainder being comprised of Type III collagen. It contains high monomer content as measured by gel permeation chromatography.

This product is supplied as a lyophilized powder with 15 mg of human collagen.  When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved.

VitroCol® is especially ideal for human cell culture systems when coating of surfaces and providing preparations of thin layers of culturing cells. VitroCol®, lyophilized form is not recommend  for the formation of a solid gel. VitroCol® human collagen is provided in user-friendly packaging for use and storage. VitroCol® is supplied as a sterile, lyophilized powder.

Parameter, Testing, and Method

VitroCol® Collagen, Lyophilized
Catalog #5008-15MG

Form

Lyophilized Powder

Package Size

15 mg

Sterilization Method

Filtration

Storage Temperature

-20°C prior to reconstitution
2 to 10°C after reconstitution

Expiration Date

Listed on product label and Certificate of Analysis

Shelf Life

24 months

Shelf Life after Reconstitution

3 months

Polyacrylamide Gel Electrophoresis
(SDS – PAGE)

> 85% collagen containing within alpha, beta,
and gamma bands < 15% collagen contained with bands traveling faster than alpha

Sterility

No growth

Endotoxin (LAL)

< 1.0 EU/ml

Cell Attachment Assay

Pass

Source

Human, Neo-Natal, Fibroblasts

 

Cell Attachment Bioassay

To demonstrate cell attachment, cells were seeded onto surfaces coated with the matrix product and onto negative control surfaces without the matrix product. Cells were seeded onto surfaces using serum-free DMEM media. All surfaces were blocked with a solution containing 1% BSA. Cells were then allowed to attach for one (1) hour at 37°C. The culture surfaces were washed with DPBS. The results indicate significant cell attachment with surfaces coated using the matrix product.
 
Without UltraPure® With UltraPure®
Human Umbilical Vein Endothelial Cells (HUVEC) 10X
VERO Epithelial Cells 10X
Human Dermal Fibroblast Cells 10X
MDCK Epithelial Cells 10X

 

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