Bovine Collagen, Type I, Lyophilized, 15 mg

Price(USD):$260.00

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Equl SKU Product Name UOM Specification Shipping Temperature Price(USD) Add to Cart:
BM-5006-15MG Bovine Collagen, Type I, Lyophilized, 15 mg EA 15 mg Gel Ice $260.00  
Introduction:

PureCol® collagen is known as the standard of all collagens for purity (>99.9% collagen content), functionality, and the most native-like collagen available.  PureCol® is isolated from bovine hides sourced from the only controlled, closed herd in the United States.  Advanced BioMatrix’s manufacturing processes comply with stringent quality standards that have proven to yield unsurpassed lot-to-lot consistency.

PureCol® collagen is approximately 97% Type I with the remainder being comprised of Type III collagen.  It is soluble atelo-collagen. It contains high monomer content as measured by gel permeation chromatography.

This product is supplied as a lyophilized powder with 15 mg of bovine collagen.  When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved.

PureCol® collagen is ideal for coating of surfaces and providing preparations of thin layers for culturing cells. PureCol®, lyophilized form is not recommended for the formation of a solid gel.  PureCol® collagen is provided in user-friendly packaging for use and storage.  This product is supplied as a sterile lyophilized powder.

 

 


Parameter, Testing, and Method

Bovine Collagen, Type I
Catalog #  5006-15MG

Form

Lyophilized Powder

Package Size

15 mg

Sterilization Method

Filtration

Storage Temperature

-20 °C prior to reconstitution
2 to 10°C after reconstitution

Expiration Date

Listed on product label and
Certificate of Analysis

Shelf Life

24 months

Shelf Life after Reconstitution

3 months

Polyacrylamide Gel Electrophoresis
(SDS – PAGE)

> 85% collagen containing within alpha, beta, and gamma bans < 15% collagen contained with bands traveling faster than alpha

Sterility

No growth

Endotoxin (LAL)

 < 1.0 EU/ml

Cell Attachment Assay

Pass

Source

Bovine Hide – Pepsin Extracted


 

 

Cell Attachment Bioassay

To demonstrate cell attachment, cells were seeded onto surfaces coated with the matrix product and onto negative control surfaces without the matrix product. Cells were seeded onto surfaces using serum-free DMEM media. All surfaces were blocked with a solution containing 1% BSA. Cells were then allowed to attach for one (1) hour at 37°C. The culture surfaces were washed with DPBS. The results indicate significant cell attachment with surfaces coated using the matrix product.
 
Without UltraPure® With UltraPure®
Human Umbilical Vein Endothelial Cells (HUVEC) 10X
VERO Epithelial Cells 10X
Human Dermal Fibroblast Cells 10X
MDCK Epithelial Cells 10X

 

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