PureCol® in DMEM/F-12 Medium, 5 mg/ml (0.5%), 35 mL

Price(USD):$416.00

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BM-5074-35ML PureCol® in DMEM/F-12 Medium, 5 mg/ml (0.5%), 35 mL EA 35 mL $416.00  
Introduction:
PureCol® EZ Gel is a ready-to-use collagen solution that forms a firm gel by simply warming to 37°C in an incubator.  The product consists of purified Type I collagen at a concentration of 0.5%, (~5 mg/ml), DMEM/F-12 medium and a mixture of L-glutamine and dipeptide (L-alanine-L-glutamine) to provide a long-lasting L-glutamine source for cell culture.

PureCol® EZ Gel is designed to improve gel consistency by pre-formulation of the medium and adjustment of the product to a neutral pH. This product avoids the inconsistencies in the preparation of the gel that can arise through variables of reagent addition, pH adjustment and handling conditions.

PureCol® EZ Gel is ideal for providing a firm gel and can also be used in the preparation of a thin layer for culturing cells.  PureCol® EZ Gel collagen is provided in a user-friendly packaging for use and storage. This product is sterile and supplied as a ready to use solution.

Parameter, Testing, and Method

PureCol® EZ Gel Collagen Solution
Catalog # 5074-G

Form

Solution

Package Size

35 mL

Storage Temperature

2-10 °C

Expiration Date

Listed on product label and
Certificate of Analysis

Concentration
(Biuret Protein Determination)

˜ 5 mg/ml (0.5%)

pH

Approx. pH 6.9 to 7.4

Gel Time
(Gel Time Assay)

< 40 minutes

Sterility

No growth

Endotoxin (LAL)

<1.0 EU/mL

Cell Attachment Assay

Pass

Collagen Source

Bovine Hide – Pepsin Extracted

Medium Supplement

DMEM/F-12 Medium

L-glutamine Source

Mixture of L-glutamine and
dipeptide (L-alanine-L-glutamine)

 

Preparation and Usage

3-D Preparation Procedure
  1. Remove PureCol® EZ Gel from 2–10°C storage. Important: To prevent gelation, maintain temperature of product at 2–10°C.
  2. Introduce PureCol® EZ Gel into cell culture system. Cells can be added to the PureCol® EZ Gel solution.
  3. To form gel, warm to 37°C. The beginning of gelation will occur within 40 minutes, but allow approximately 90 to minutes for firm gel formation.

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