Bovine Collagen Solution, Type I, 3 mg/ml, 100 ml

Price(USD):$611.00

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BM-5005-100ML Bovine Collagen Solution, Type I, 3 mg/ml, 100 ml EA 100 ml Gel Ice 2 $611.00  
Introduction:

PureCol® collagen is known as the standard of all collagens for purity (>99.9% collagen content), functionality, and the most native-like collagen available. PureCol® is isolated from bovine hides sourced from the only controlled, closed herd in the United States. Advanced BioMatrix’s manufacturing processes comply with stringent quality standards that have proven to yield unsurpassed lot-to-lot consistency.

PureCol® collagen is approximately 97% Type I collagen with the remainder being comprised of Type III collagen. It contains a high monomer content as measured by gel permeation chromatography. PureCol® collagen is supplied at approximately a 3 mg/ml concentration. The concentration for each specific lot is provided on a Certificate of Analysis that is available with the purchase of each product. PureCol® is soluble atelo-collagen in 0.01 N HCI, therefore, the pH is approximately 2.0. PureCol® collagen is ideal for coating of surfaces, providing preparation of thin layers for culturing cells, or use as a solid gel.

PureCol® collagen is provided in a user-friendly packaging for use and storage. PureCol® is sterile filtered and is supplied as a ready to use solution.

 

Parameter, Testing, and Method

PureCol® Collagen Solution
Catalog # 5005-100ml

Sterilization Method

Filtration

Form

Solution

Package Size

100 ml

Storage Temperature

2-10 °C

Expiration Date

Listed on product label and
Certificate of Analysis

Concentration
(Biuret Protein Determination)

2.9-3.2 mg/ml

Purity
(collagen concentration)

> 99.9%

pH

Approx. pH 2, 0.01 N HCl

Gel Time
(Gel Time Assay)

< 40 minutes

Fibrillogenesis
(Fibril Formation)

>0.5 Absorbance Units

Polyacrylamide Gel Electrophoresis
(SDS – PAGE)

> 85% collagen containing within alpha, beta,
and gamma bands < 15% collagen contained with bands traveling faster than alpha

Sterility

No growth

Endotoxin (LAL)

< 1.0 EU/ml

Osmolality

~ 20 mOsm

Conductivity

~ 2300 µs/cm

Cell Attachment Assay

Pass

Source

Bovine Hide – Pepsin Extracted


 

 

Cell Attachment Bioassay

To demonstrate cell attachment, cells were seeded onto surfaces coated with the matrix product and onto negative control surfaces without the matrix product. Cells were seeded onto surfaces using serum-free DMEM media. All surfaces were blocked with a solution containing 1% BSA. Cells were then allowed to attach for one (1) hour at 37°C. The culture surfaces were washed with DPBS. The results indicate significant cell attachment with surfaces coated using the matrix product.
 
Without UltraPure® With UltraPure®
Human Umbilical Vein Endothelial Cells (HUVEC) 10X
VERO Epithelial Cells 10X
Human Dermal Fibroblast Cells 10X
MDCK Epithelial Cells 10X

 

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