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DEAE Cellthru BigBead Plus

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EQUL-BSM31 DEAE Cellthru BigBead Plus EA call for price none
Introduction:
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Product Code11020
Bead Size300-500µm
Matrix Structure4% agarose, hardened
Flow rate5000cm/h*
Binding Capacity50mg BSA/ml resin
Cleaning0.5M NaOH
Storage Conditions20% ethanol, 2-8C
Temperature stability range0°- 60°C
Functional ligand-N+(C2H5)2
Ion Exchanger TypeWeak anion
Counter ionCl-
pH stability range2-14
Chemical stability0.5M NaOH, 8M urea, 6M guanidine, ethanol and common buffers
Physical stabilityNegligible volumbe changes due to pH or Ionic state
Drug master fileU.S. Food & Drug Administration

*Flow rate determined in a 1.5x5cm column



- Large particle diameter allows crude material to pass through the column with little to no backpressure during direct capture
- Four different ion exchange chemistries can be used with a variety of bead sizes
- Robust, chemically stable beads are ideal for process-scale separations
- Product recovery, up to 95%
-cGMP manufacturing; Regulatory Support File available
-DMF with USFDA

Ion exchange Cellthru BigBead Plus resins are made of a hardened agarose matrix with ion exchange groups attached through a spacer arm. Hardening increases the chemical and mechanical stability of the matrix. DEAE Cellthru BigBead Plus is a large particle, weak anion exchanger, where capacity is a function of pH.

Cellthru BigBeads can be used for viscous feed streams and direct capture in standard packed-bed applications using specially manufactured columns. Scale up from pilot to production scale follows a linear relationship, reducing the time spent on method development.