IDA Chelating Ultraflow 4

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Equl SKU Product Name UOM Price(USD) Add to Cart:
EQUL-BSM21 IDA Chelating Ultraflow 4 EA call for price none
Product Code37411
Bead Size40-160µm
Agarose concentration4%
Flow rate680cm/h*
Binding Capacity>40mg hemoglobin/ml
Cleaning0.5M NaOH
Storage Conditions20% ethanol, 2-8C
Reactive group concentration16µmole/ml
Temperature stability range0-60 C
Functional ligandIminodiacetic acid
pH stability range2-14
Chemical stabilityHigh salt, 0.1M NaOH (<2hr), 6M guanidine, 70% ethanol
Drug master fileU.S. Food & Drug Administration

*Flow rate determined in a 1.5x5cm column

- For purification of heme-containing proteins such as hemoglobin or tagged proteins whose tags have affinity for divalent metals (Ni, Co, Cu, Zn, Fe, etc.).
- High binding capacity with low non-specific binding
- Iminodiacetic acid immobilized on Sterogene’s patented Ultraflow 4 matrix
- Cleanable and industrial process-ready
- No detectable leaching
- cGMP manufacturing; Regulatory Support File available
- DMF with USFDA

IDA Chelating Ultraflow 4 is an immobilized metal affinity chromatography (IMAC) resin that separates proteins via their differences in affinity to various metal ions (i.e., Ni, Fe, Co, Cu, or Zn).

A number of commercially available metal-chelating resins differ by how strongly they bind a given metal. For instance, NTA resin is a tetra-dentate chelator, leaving two coordination sites available on a transition metal to bind chelating moieties on proteins (such as histidine repeats).

Sterogene’s IDA Chelating resins are tri-dentate and typically have high binding capacities in comparison to other chelators. IDA Chelating Ultraflow 4 is shipped uncharged so that the customer can select which transition metal to chelate with the resin. Typically, users find Ni, Fe, Co, Cu, or Zn the most effective. Optimizing the separation usually requires some method development.

Also Consider
Note that IDA is not an ideal chelating resin for purifying His-tagged proteins; we recommend either Nickel Chelating Superflow or Cobalt Chelating Superflow for these proteins.