Glutathione Affinity Superflow

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EQUL-BSM20 Glutathione Affinity Superflow EA call for price none
Product Code37009
Bead Size60-160µm
Agarose concentration6%
Flow rate1400cm/h*
Binding Capacity10mg GST-tagged protein/ml
Cleaning0.1M NaOH for 30min
Storage Conditions20% ethanol, 2-8C
Reactive group concentration32µmole/ml gel
Temperature stability range0-60 C
Functional ligandGlutathione
pH stability range3-12
Chemical stabilityHigh salt, 0.1M NaOH (<2hr), 6M guanidine, 70% ethanol
Drug master fileU.S. Food & Drug Administration

*Flow rate determined in a 1.5x5cm column

- For binding of GST-tagged proteins
- High binding capacity with low non-specific binding
- Performance is on par with leading immobilized glutathione resins
- Immobilized on Sterogene’s own patented Superflow matrix (Most industry leading tagged fusion protein binding resins use Sterogene’s Superflow matrix.)
- Cleanable and industrial process-ready

- cGMP manufacturing; Regulatory Support File available
- DMF with USFDA

Sterogene manufactures Superflow beads, and has also developed tagged purification beads that perform well compared with the market leaders in tagged protein purification. Superflow is used by many tagged purification resin suppliers today because of its low nonspecific binding properties. These provide effective and efficient one-step purifications in many different expression systems.

Glutathione Affinity Superflow is made by optimized immobilization of high-grade glutathione to Sterogene’s trademarked Superflow 6 matrix. Glutathione/GST purification has proven to be a simple, effective method of purifying recombinant proteins expressed in the pGEX vector. Once bound to the resin, the GST-tagged protein can be eluted with glutathione or the GST can be cleaved from the protein while still on the column, eluting the GST-free recombinant protein.