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Nickel Chelating Superflow

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EQUL-BSM18 Nickel Chelating Superflow EA call for price none
Introduction:
Product Code37408
Bead Size60-160µm
Agarose concentration6%
Flow rate1800cm/h*
Binding Capacity5-10mg/ml of 6X His-tagged protein
Cleaning0.5M NaOH for 30min
Storage Conditions20% ethanol, 2-8C
Reactive group concentration24 µmole Ni/ml gel
Temperature stability range0-60 C
Functional ligandProprietary
LeachingNegligible
pH stability range2-13(<2hr) or 3-12 (>2hr)
Chemical stabilityHigh salt, 0.5M NaOH (<2hr), uea, guanidine, organic solvents: ethanol (70%), methanol (50%)
Drug master fileU.S. Food & Drug Administration

*Flow rate determined in a 1.5x5cm column



- For binding of His-tagged proteins
- High binding capacity with low non-specific binding
- Performance is on par with leading Ni-chelating resins
- Immobilized on Sterogene’s own patented Superflow matrix (Most industry leading chelators use Sterogene’s Superflow matrix.)
- Cleanable, rechargeable and industrial process-ready
- No detectable leaching
- cGMP manufacturing; Regulatory Support File available
- DMF with USFDA

Nickel Chelating Superflow is an immobilized metal affinity chromatography (IMAC) resin that separates proteins via their differences in affinity to nickel. This product is recommended as a drop in replacement for other tetra-dentate chelators.

A number of commercially available metal-chelating resins differ by how strongly they bind a given metal. For instance, Sterogene’s IDA Chelating resins are tri-dentate and typically have high binding capacities in comparison to other chelators. However, relative to tetra-dentate chemistries, tri-dentate chelators tend to have higher metal leakage properties. For this reason, tri-dentate chelators such as IDA are not ideal for binding His-tagged proteins.

NTA resin is a tetra-dentate chelator, leaving two coordination sites available on a transition metal to bind chelating moieties on proteins (such as histidine repeats).Nickel Chelating Superflow features a proprietary tetra-dentate chelating chemistry (not NTA) optimized for binding His-tagged proteins with nickel.
The product is shipped pre-charged and ready to use. Optimizing the separation usually requires some method development.