Actigel T

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EQUL-BSM13 Actigel T EA call for price none
Product Code2703
Bead Size60-160um
Agarose concentration4%
Molecular exclusion limit20 million Daitons
Spacer arm12 atoms, hydrophilic
Flow rate250cm/h*
CleaningOrganic solvents: 50% ethanol, methanol
Storage conditions20% ethanol, 2-8C
Functional group to attachThiol containing molecules
Reactive group concentration30-45umoles/ml resin
Temperature stability rangeAmbient
Functional ligandThiopyrdyl
Blocking agentsNot applicable
Coupling in organic solventsYes
Coupling in a packed columnYes
pH stability range2-8
Coupling conditionspH 7-10, at 4-22C for 10min to 1hour
Chemical stability8M urea, 6M guanidine, ethanol and common buffers
Drug master fileU.S. Food & Drug Administration

*Flow rate determined in a 1.5x5cm column

-For reversible coupling of thiol-containing proteins and subsequent selective elution based on relative thiol reactivity
-High subsititution levels: immobilizations can easily reach 20mg immbolizied protein per ml resin
-Utilizes Sterogene's patented Ultraflow 4 matrix
-Cleanable and industrial process-ready -cGMP manufacturing; Regulatory Support File available

Actigel T is used for the reversible coupling of thiol-containing ligands and/or the purification of thiol-containing molecules. Appropriate ligands for immobilization to Actigel T include small molecules, peptides or proteins.

During the reaction, a thiol-disulfide exchange takes place, attaching the ligand to the resin through a disulfide bond. This bond can then be cleaved by a reducing agent such as mercaptoethanol or dithiothreitol, liberating the ligand.

This strategy is useful when the molecule of interest has a reactive thiol group, while others thiols in the sample are less active. The molecule of interest binds preferentially to Actigel T, leaving other components of the smaple in solution. Unbound proteins are removed in the wash step, after which bound protein/ligand is cleaved form the resin by a reducing agent.