DNA Etox

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EQUL-BSM03 DNA Etox EA call for price none
Product Code2706
Bead Size60-160um
Agarose concentration4%
Flow rate2400cm/hr*
Binding capacity>500,000EU/ml resin (in a plasmid preparation)
Cleaning1N NaOH for 2-16 h
Storage Conditions20% ethanol, 2-8C
Cycle times100
Temperature stability range0-60C
Functional ligandNontoxic and nonmutagenic (proprietary)
pH stability range2-14
Chemical stability1M NaOh, 8M urea, 6M guanidine, ethanol, and common buffers. Incompatible with heavy metals.
Drug master fileU.S. Food & Drug Administration

*Flow rate determined in a 1.5x5cm column

-For removal of endotoxin present in RNA, DNA and oligonucleotide preparations
-Removes endotoxin to below 0.1EU/ml (may require multiple passes through column)
-High affinity for endotoxin with low non-specific binding; delivers a mass yield of DNA that typically exceeds 90%
-Binds the lipid-A portion of endotoxin in a mixed mode biomimetic affinity interaction
-Cleanable with 1M NaOH (an industry standard for destruction of endotoxin)
-Can be sanitized and reused up to 100 times with minimal loss of binding efficancy
-No detectable leaching
-Nontoxic ligand
-cGMP manufacturing; Regulatory Support File available

Removing endotoxin from DNA is often difficult because of the physicochemical similarities of DNA and endotoxin. The most commonly used methods (ul trafiltration, size exclusion and ion exhange chromatography) have limited utilty in this area. Consequently, endotoxin removal is typically tedious and can incur significant losses of DNA during the process. Sterogene's DNA Etox overcomes these difficulties.

DNA Etox utilizes Sterogene's proprietary ALD coupling chemistry, an immobilization technique that produces a highly stable secondary amine linkage. Leaching is negligible, less then 0.1ppm, the sensitivity limit of the assay.

DNA Etox can be used in either a batch mode or a standard chromatography column. In batch mode, the settled gel is simply added directly to the DNA solution and vortexed. Serveral contact times ranging from 3 min to 20 min should be tested to achieve the most complete removal of endotoxin.