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RIDASCREEN® CIS-Casein

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R5102 RIDASCREEN® CIS-Casein Microtiter plate, (6 strips with 8 removable wells call for price none
Introduction:

Intended use:
RIDASCREEN® CIS-Casein is a competitive enzyme immunoassay for the analysis of bovine casein in ewes’ and goats’ milk, cheese and yogurt.

General information:
The production of ewes’ and goats’ cheese is of economical importance in the
European Community, especially for France, Spain, Italy and Greece.
But as the production of ewes’ and goats’ milk depends on the season of the year, getting over the restricted production with the addition of cows’ milk in cheese production is of economical interest.
In addition to the reference method (Reg. No. 213/2001) of the European Commission, in which the isoelectric focusing of bovine γ-casein is recommended, the RIDASCREEN® CIS-Casein assay provides a method for more specific and sensitive bovine γ-casein detection in milk, cheese and yogurt.
In comparison to the isoelectric focusing, which is time consuming and requires special equipment, the ELISA is easy to handle, very sensitive and also very specific.


Specifications:
Format:Microtiter plate, (6 strips with 8 removable wells each)
Standards:0 %, 0.5 %, 1 % bovine casein
Sample preparation: homogenization and extraction
centrifugation / drying (acetone)
Incubation time:2 h 30 min
Detection limit: chese: 0.5 % bovine casein
Cross-reactions: The antibodies used in the RIDASCREEN® CIS-Casein assay are monospecific against bovine γ-casein. The antibodies recognize bovine γ1-, γ2-, γ3- und β-caseins to a similar extent.
No cross reactivity of the purified antibodies against ewes’ and goats’ milk protein fractions were observed, but the antibodies cross react with buffalo casein.
The test must therefore not be applied for the detection of cows’ milk products containing buffalos’ milk.