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RIDASCREEN® Sulfonamide

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R3004 RIDASCREEN® Sulfonamide Microtiter plate with 96 wells (12 strips with 8 r call for price none
Introduction:

Intended use:
RIDASCREEN® Sulfonamide is a competitive enzyme immunoassay for the quantitative analysis of sulfonamides in egg, meat (chicken and pork), fish, shrimps, honey and milk.

General information:
Sulfonamides are widely used as feed additives, mainly for fattening of calves and
pigs. Combined with inhibitors of dihydrofolate reductase such as trimethoprim,
tetroxoprim, or pyrimethamine sulfonamides are also used in veterinary medicine for the treatment of intestinal infections, mastitis, pulmonitis and other (systemic) diseases. Therefore sulfonamide residues may occur in food of animal origin such as meat and milk and were found frequently abroad. According to the EU-Law a maximum residue limit for all substances of the sulfonamide-group of 100 ppb in muscle, fat, liver and kidney and of 100 ppb in milk is valid.


Specifications:
Format:Microtiter plate with 96 wells (12 strips with 8 removable wells each)
Standards:0 ppb (zero standard), 1 ppb, 3 ppb, 10 ppb, 30 ppb, 100 ppb
Sample preparation: milk: after 1:5 dilution direct use in the assay

meat (chicken and pork), egg, fish, shrimps:
homogenization, extraction, centrifugation, evaporation
and degreasing

honey: extraction, RIDA® C18 column purification and
dilution
Incubation time:1 h 15 min
Detection limit: meat (chicken), egg: 1.5 ppb
meat (pork), fish, shrimps, honey: 2 ppb
milk: 3.5 ppb
Cross-reactions: Sulfamethoxypyridazine: approx. 100 %
Sulfapyridine: > 100 %
Sulfamethoxydiazine: approx. 75 %
Sulfamethoxazole: approx. 58 %
Sulfadimethoxine: approx. 41 %
Sulfaquinoxaline: approx. 34 %
Sulfathiazole: approx. 32 %
Sulfachloropyridazine: approx. 19 %
Sulfamerazine: approx. 18 %
Sulfadiazine: approx. 15 %
Sulfamethizole: approx. 14 %
Sulfadoxine: approx. 10 %
Sulfachloropyrazine: approx. 9 %
Sulfaguanidine: 5 %
Sulfaphenazole, Sulfamethazine,
Sulfisoxazole, Sulfanilamide: 2 %
Sulfacetamide: 1 %