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RIDASCREEN® Streptomycin

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R3103 RIDASCREEN® Streptomycin Microtiter plate with 96 wells (12 strips with 8 r call for price none
Introduction:

Intended use:
RIDASCREEN® Streptomycin is a competitive enzyme immunoassay for the quantitative analysis of streptomycin and dihydrostreptomycin in milk, honey, meat and liver.

General information:
Next to the ß-lactam antibiotics in veterinary medicine streptomycin is one of the mostly used antibiotics for the treatment of mastitis. Residues of streptomycin may therefore occur in food of animal origin, if the withholding period is not obeyed or if it is used improperly. High concentrations of streptomycin show ototoxic and nephrotoxic effects. The chronic exposure of humans with low concentrations, as found in food, may cause allergies, impair the intestinal flora and induce resistance of pathogenic microorganisms. To protect the consumer against healthy risks and avoid food-technological problems, a sensitive and simple method for the detection of streptomycin is necessary. The EU-regulations for streptomycin are fixed in MRLs (maximum residue limits) for meat and milk (muscle and liver: 500 ppb, kidney: 1000 ppb and milk: 200 ppb). For honey Germany fixed also a limit of 20 ppb.


Specifications:
Format:Microtiter plate with 96 wells (12 strips with 8 removable wells each)
Standards:0 ppb (zero standard), 0.5 ppb, 1.5 ppb, 4.5 ppb,
13.5 ppb, 40.5 ppb
Sample preparation: milk: dilution
honey: extraction, RIDA® C18 column purification,
evaporation and reconstitution in buffer
meat: homogenization, centrifugation, precipitation
and degreasing
Incubation time:1 h 15 min
Detection limit: honey: approx. 5 ppb
milk: 10 ppb
meat: 20 ppb
liver: 25 ppb

(corresponding to the standard substance)
Cross-reactions: Streptomycin: 100 %
Dihydrostreptomycin: 149 %