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RIDASCREEN® Acetylgestagene

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R1801 RIDASCREEN® Acetylgestagene Microtiter plate with 96 wells (12 strips with 8 r call for price none
Introduction:

Intended use:
RIDASCREEN® Acetylgestagene is a competitive enzyme immunoassay for the quantitative analysis of medroxyprogesterone acetate (MPA) and other acetylgestagenes in bovine perirenal fat.

General information:
Medroxyprogesterone acetate (MPA), 17α-acetoxyprogesterone, megestrol acetate and chlormadinone acetate belong to the group of acetylgestagenes. Acetylgestagenes are known to increase the efficiency of animal production and have been used in illegal cocktails for calf fattening in the past. However these substances are not permitted as resources for animal fattening.
Some studies have shown that the peak-concentrations of acetylgestagene-residues
can be observed in fat tissue. MPA, for instance, can be detected in plasma only a few days past application but it cannot be detected with accuracy in urine. After the application of MPA to calfs high concentrations of non-metabolized MPA can be detected in fat, even after 19 days.


Specifications:
Format:Microtiter plate with 96 wells (12 strips with 8 removable wells each)
Standards:0 ppb (zero standard), 0.15 ppb, 0.45 ppb, 1.35 ppb,
4.05 ppb, 12.15 ppb
Sample preparation: extraction over night, centrifugation, evaporation, freezing, centrifugation and chromatographic purification with RIDA® C18 columns (Art. No.: R2002)
Incubation time:2 h 30 min
Detection limit: Medroxyprogesterone acetate: 0.3 ppb
17α-Acetoxyprogesterone: 0.24 ppb
Megestrol acetate: 0.6 ppb
Chlormadinone acetate: 0.6 ppb

(corresponding to the standard substance)
Cross-reactions: Medroxyprogesterone acetate: 100 %
17α-Acetoxyprogesterone: 126 %
Megestrol acetate: 50 %
Chlormadinone acetate: 50 %
Melengestrol acetate: 0.6 %
cross reaction of all other substances being checked in the assay: ≤ 0.002 %