T-2 Immunoaffinity Column (IAC)

Price(USD):$352.04

       *Ask Promo Code:    
Equl SKU Product Name UOM Price(USD) Add to Cart:
T2IAC-1ML T-2 Immunoaffinity Column (IAC) 1ml/pcs,25pcs/box $352.04  
Introduction:

T-2 Immunoaffinity Column (IAC)

Cat.# T2IAC
Intended use
The application of IAC on the complicated sample matrix will facilitated the purification of T2 in food and feed, After this step, the T2 can be later quantitative analysis by TLC, HPLC ,GC ,LC-MS/MS, EIA.
Capacity: above 200 ng of T2
Storage Conditions: IAC should be stored at 2-8℃. Do not freeze.

Use
Clean up of sample extracts for further analysis.

summary
T2 is a mycotoxin produced by bacteria from a variety sickle. Contaminated food crops such as wheat, barley, corn.T2 affects the blood, liver, kidney ,pancreas, muscles and lymphocyte function ,constitute a greater hazard to human health and animal husbandry. most countries have established regulatory limits on T2 in food and feed.

Use Principles
The IAC is based on the reaction between antigen and antibody. This T2 IAC contains gel binding with T2 antibody. The T2 in the samples will be extracted, filtered, diluted and passed through the T2 IAC. T2 in the extract will be retained in the IAC by the T2 specific antibody, the impurity in the extract will pass through. After rinsing to completely remove the unwanted matrix, the T2 is eluted by Methanol by de-associating the T2 from the antibody. This elute will be ready for instrumental analysis.

Materials Provided
25 disposable IAC per box, 1ml each
Instructions

Precautions
T2 are toxic substance. please wear gloves to go experimental operation.
Used lab wares and T2 solution preferably be soaked with 5℅ sodium hypochlorite solution soak over night.
Do not use the reagents after the expiration date.
IAC stored at 2-8℃,no not freeze
Reagents should be brought to room temperature (22-25℃) prior to use. Avoid prolonged (>24 hours) storage at room temperature.

Operation Procedure
Grind the sample in a blender and sieve through # 20 sieve. Sample will not be extracted right away should be store in the refrigerator.
Weigh 20 gram of sample and mix with 4 gram of NaCl, then add 100 ml of 70% Methanol in water.
Homogenize for 2 minute or shake for 30 minute.
Filter at least 5 ml of the extract through paper filter.
Transfer 5ml of filtrate and mix with 20 ml of water.
Filter this diluted extract through a microfiber filter, Load 5 ml of this clear solution onto the IAC
Connect 10 ml of syringe barrel reservoir to the column.
Load the column with 5 ml diluted and filtered extract and control the flow rate at 1-2 drops per second. Discard the pass through liquid
Use 10ml of water to rinse the column and discard the rinse.
Press the air into the column to blow out all the liquid
Use 1 ml of methanol to elute the sample (flow at one drop/2-3 seconds).
collect the sample for further testing.