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Aflatoxin B1 Immunoaffinity Column (IAC)

Price(USD):$270.79

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B1IAC-1ML Aflatoxin B1 Immunoaffinity Column (IAC) 1ml/支,25支/盒 $270.79  
Introduction:

Aflatoxin B1 Immunoaffinity Column (IAC)

Cat.# B1IAC

Intended use
The application of IAC on the complicated sample matrix will facilitated the purification of AflatoxinB1 in food and feed, After this step, the Aflatoxin B1 can be later quantitative analysis by TLC, HPLC ,GC ,LC-MS/MS, EIA.
Capacity: above 300 ng of Aflatoxin B&G
Storage Conditions: IAC should be stored at 2-8℃. Do not freeze.

Use
Clean up of sample extracts for further analysis.

summary
As the common mildew fungus of cereals and other crops, Aflatoxin is a class of low molecular weight compounds produced by Aspergillus flavus and Aspergillus parasiticus. Contamination grain, oil crops seed, especially peanuts, corn is the most susceptible to contamination, another is often detected in wheat, sorghum, Brazil nuts, beans and rice and other important food.
Aflatoxin would be difficult to remove once produced because of high thermal stability. Highly toxic, carcinogenic strongest of animals, plants and micro-organisms, can cause mutations and lead to deformity.
As one of the three causes of liver cancer, Aflatoxin is gastric cancer, colorectal cancer and other diseases posing a great threat to human health. Countries in the world have strict limits on Aflatoxin in food, feed.

Use Principles
The IAC is based on the reaction between antigen and antibody. This Aflatxoin B1 IAC contains gel binding with Aflatoxin antibody. The AFB1 in the samples will be extracted, filtered, diluted and passed through the Aflatoxin B1 IAC. Aflatoxin B1 in the extract will be retained in the IAC by the Aflatoxin specific antibody, the impurity in the extract will pass through. After rinsing to completely remove the unwanted matrix, the AFB1 is eluted by Methanol by de-associating the AFB1 from the antibody. This elute will be ready for instrumental analysis.

Materials Provided
25 disposable IAC per box. 1ml each
Instructions

Precautions
Aflatoxins are toxic substance. please wear gloves to go experimental operation.
Used lab wares and Aflatoxin solution preferably be soaked with 5℅ sodium hypochlorite solution soak over night.
Do not use the reagents after the expiration date.
IAC stored at 2-8℃,no not freeze.
Reagents should be brought to room temperature (22-25℃) prior to use.

Operation Procedure
Grind the sample in a blender and sieve through # 20 sieve. Sample will not be extracted right away should be store in the refrigerator.
Weigh 20 gram of sample and mix with 4 gram of NaCl, then add 100 ml of 70% Methanol in water.
Homogenize for 2 minute or shake for 30 minute.
Filter at least 10 ml of the extract through paper filter.
Transfer 10 ml of filtrate and mix with 20 ml of water.
Filter this diluted extract through a microfiber filter, Load 15 ml of this clear solution onto the IAC.
Connect 10 ml of syringe barrel reservoir to the column.
Load the column with 15 ml diluted and filtered extract and control the flow rate at 1-2 drops per second. Discard the pass through liquid.
Use 10 ml of water to rinse the column and discard the rinse.
Press the air into the column to blow out all the liquid
Use 1 ml of methanol to elute the sample (flow at one drop/2-3 seconds).
Collect the sample for further testing.