Ochratoxin Immunoaffinity Column (IAC)


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ZEAIAC-1ML Ochratoxin Immunoaffinity Column (IAC) 1ml/pcs,25pcs/box $352.04  

Ochratoxin Immunoaffinity Column (IAC)
Cat.# OIAC

Intended use
The application of IAC on the complicated sample matrix will facilitated the purification of Ochratoxin in food and feed, After this step, the Ochratoxin can be later quantitative analysis by TLC, HPLC ,GC ,LC-MS/MS, EIA.
Capacity: above 300 ng of Ochratoxin
Storage Conditions: IAC should be stored at 2-8℃. Do not freeze.

The sample extracts for further analytical instruments.

Ochratoxin including seven kinds of structurally similar compounds, one of the most toxic of Ochratoxin A in moldy grain, feed, ect. The most Common, this toxic is the main cause of kidney damage, a lot of toxins may also cause the animal intestinal mucosal inflammation and necrosis, constitute a greater hazard to human health and animal husbandry, countries around the world have strict limits for ochratoxin in food.

Use Principles
The IAC is based on the reaction between antigen and antibody. This Ochratoxin IAC contains gel binding with Ochratoxin antibody. The Ochratoxin in the samples will be extracted, filtered, diluted and passed through the Ochratoxin IAC. Ochratoxin in the extract will be retained in the IAC by the Ochratoxin specific antibody, the impurity in the extract will pass through. After rinsing to completely remove the unwanted matrix, the Ochratoxin is eluted by Methanol by de-associating the Ochratoxin from the antibody. This elute will be ready for instrumental analysis.

Materials Provided
25 disposable IAC per box, 1ml each

Ochratoxin are toxic substance. please wear gloves to go experimental operation.
Used lab wares and Ochratoxin solution preferably be soaked with 5℅ sodium hypochlorite solution soak over nigh.
Do not use the reagents after the expiration date.
IAC stored at 2-8℃,no not freeze.
Reagents should be brought to room temperature (22-25℃) prior to use.

Operation Procedure 
Grind the sample in a blender and sieve through # 20 sieve. Sample will not be extracted right away should be store in the refrigerator.
Weigh 20 gram of sample and mix with 4 gram of NaCl, then add 100 ml of 70% Methanol in water.
Homogenize for 2minute or shake for 30 minute.
Filter at least 5 ml of the extract through paper filter.
Transfer 5ml of filtrate and mix with 20 ml of water.
Filter this diluted extract through a microfiber filter, Load10ml of this clear solution onto the IAC.
Connect 10 ml of syringe barrel reservoir to the column.
Load the column with 10 ml diluted and filtered extract and control the flow rate at 1-2 drops per second. Discard the pass through liquid.
Use 10ml of water to rinse the column and discard the rinse.
Press the air into the column to blow out all the liquid
Use 1.5 ml of methanol to elute the sample (flow at one drop/2-3 seconds),Slowly blow all methanol out collect the sample for further testing.