Gelfree 8100 Fractionation Station


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481001 Gelfree 8100 Fractionation Station EA Unit + Cartridges $31,200.00  

Sample complexity necessitates extensive fractionation for in-depth proteomic analysis using mass spectrometry. Protein Discovery, now part of the Expedeon group, has developed a commercial system using a novel technology, termed Gel Elution Liquid Fraction Entrapment Electrophoresis (GELFREE), for molecular weight-based fractionation of intact proteins with liquid phase recovery. Eight samples are run in parallel using a programmable control module in ca. 90 minutes. The system is shown to provide robust fractionation over the mass range 3.5 kDa - 500 kDa with high loading capacity, reproducibility, and recovery.


The Gelfree 8100 Fractionation System for mass spectrometry sample preparation partitions complex protein mixtures into userselectable liquid-phase molecular weight fractions.

Additional Information

Shelf Life Cartridges = Minimum 3 Months
Storage Room Temperature


 Intact protein molecular weight fractionation, isolation, and purification

• Liquid-phase recovery without band or spot cutting

• Broad mass range fractionation up to 500 kDa

• Up to eight samples processed in parallel

• Programmable fractionation for isolating and purifying targeted proteins

• High protein recovery (>80%)

• High reproducibility (<15% CV)

• Sampling unbiased by hydrophobicity, pI

• High loading capacity (>5X more than a 1D gel)

• Proteins are recovered intact, for complete characterization



• Simplify and reduce the dynamic range of complex protein mixtures for bottom-up discovery proteomics using LC-MS/MS

• Fractionate and recover proteins intact for top-down proteomics

• Isolate and enrich user-selected molecular weight fractions for targeted protein quantification using LC-MS/MS

• Isolate intact proteins to analyze variants, post-translational modifications, alterations

• Separate protein pull-down components for target protein purification

• Separation, isolation, and intact recovery of antibodies intact for in-depth characterization



The Gelfree 8100 Fractionation System consists of the Gelfree 8100 Fractionation Station and ready-to-use Gelfree 8100 Cartridges.