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  • Megazyme Hydrogen Peroxide Assay Kit (Megaplex Red)  K-MRH2O2
Megazyme Hydrogen Peroxide Assay Kit (Megaplex Red)  K-MRH2O2

Megazyme Hydrogen Peroxide Assay Kit (Megaplex Red) K-MRH2O2

  • SKU: K-MRH2O2
  • Brand: Megazyme
  • Price: USD$350
  • Size: 1000 assays (microplate) / 100 assays (manual) / 400 assays (auto-analyser)
  • Product description: Hydrogen Peroxide Assay Kit (Megaplex Red)
  • Email: info@equl.com
Content: 1000 assays (microplate) / 100 assays (manual) / 400 assays (auto-analyser)
Shipping Temperature: Ambient
Storage Temperature: Short term stability: 2-8oC,
Long term stability: See individual component labels
Stability: > 2 years under recommended storage conditions
Analyte: Hydrogen Peroxide
Assay Format: Spectrophotometer, Microplate, Auto-analyser
Detection Method: Absorbance, Fluorescence
Wavelength (nm): (Fluorescence) 530-560 (ex), ~ 590 (em)
(Absorbance) 570
Signal Response: Increase
Linear Range: (Fluorescence) 0.0034-0.17 µg of H2O2 per assay (0.1-5 µM in-assay)
(Absorbance) 0.034-0.68 µg of H2O2 per assay (1-20 µM in-assay)
Limit of Detection: (Fluorescence) 0.0034 ug/mL
(Absorbance)
0.25 ug/mL
Reproducibility (%): (Fluorescence) ~ 8%
(Absorbance) ~ 8%
Reaction Time (min): (Fluorescence) 30 min
(Absorbance)
15 min
Application examples: Food and beverage samples such as milk or milk based products, wine, fruit juices, paper (and cardboard), waste treatment samples and other materials (e.g. biological cultures etc.).

The hydrogen peroxide Assay Kit provides a simple robust method for the measurement of hydrogen peroxide. This kit utilizes a highly sensitive Megaplex Red probe which in the presence of horseradish peroxidase (HRP), allows for the measurement of H2O2 in a sample. Upon oxidation of the probe by HRP, a coloured product (resorufin) is formed that can be measured in fluorescence mode using excitation in the range 530-560 nm and emission at ~ 590 nm or colourimetrically at 570 nm. The excitation and emission maxima of Megaplex Red are 571 nm and 585 nm respectively.